Cell lines have been used as excellent in vitro models for research of neural process, drug discovery, and toxicity screening testing due to their many advantages:
neural cell lines originated from a single ancestor cell provide a homogeneous population of neurons; they have the capability of long-term growth and ease to cultivate; they provide unlimited materials for the development of in vitro models; they can grow and differentiate into neuron-like cells or tissues by controlling culture conditions; they are more easily transfected compared to primary neurons. The in vitro neural models induced from neural cell lines have been shown to express neuronal markers and possess the functions of mammalian neurons in vivo, such as possessing axons and dendrites as well as electrical excitability.
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